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Last updated: 2016/10/16
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CYP24A1 monoclonal antibody (M02), clone 1E1

  • Catalog # : H00001591-M02
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against a partial recombinant CYP24A1.
  • Immunogen:
  • CYP24A1 (NP_000773, 415 a.a. ~ 514 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
  • Sequence:
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Isotype:
  • IgG2a Kappa
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.

    QC Testing of H00001591-M02
    Western Blot detection against Immunogen (36.74 KDa) .
  • Storage Buffer:
  • In 1x PBS, pH 7.4
  • Storage Instruction:
  • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot analysis of CYP24A1 expression in transfected 293T cell line by CYP24A1 monoclonal antibody (M02), clone 1E1.

    Lane 1: CYP24A1 transfected lysate (Predicted MW: 11.11 KDa).
    Lane 2: Non-transfected lysate.
  • PDF DownloadProtocol Download
  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • Detection limit for recombinant GST tagged CYP24A1 is approximately 1ng/ml as a capture antibody.
  • PDF DownloadProtocol Download
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 1591
  • Gene Name:
  • CYP24A1
  • Gene Alias:
  • CP24,CYP24,MGC126273,MGC126274,P450-CC24
  • Gene Description:
  • cytochrome P450, family 24, subfamily A, polypeptide 1
  • Gene Summary:
  • This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • 1,25-@dihydroxyvitamin D3 24-hydroxylase,24-OHase,OTTHUMP00000031314,cytochrome P450 family 24 subfamily A polypeptide 1,cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase),exo-mitochondrial protein,vitamin D 24-hydroxylase
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