CYP11A1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CYP11A1 full-length
Host
Human
Theoretical MW (kDa)
60.1
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CYP11A1 antibody (H00001583-B01) by Western Blots.
SDS-PAGE Gel
CYP11A1 transfected lysate.
Western Blot
Lane 1: CYP11A1 transfected lysate ( 60.1 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CYP11A1
Entrez GeneID
1583GeneBank Accession#
BC032329.1Protein Accession#
-Gene Name
CYP11A1
Gene Alias
CYP11A, P450SCC
Gene Description
cytochrome P450, family 11, subfamily A, polypeptide 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide. [provided by RefSeq
Other Designations
cholesterol 20-22 desmolase|cholesterol monooxygenase (side-chain cleaving)|cytochrome P450, subfamily XIA|cytochrome P450, subfamily XIA (cholesterol side chain cleavage)|cytochrome P450C11A1|steroid 20-22-lyase
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Interactome
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Pathway
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Disease
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