CYP3A7 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CYP3A7 full-length
Host
Human
Theoretical MW (kDa)
57.5
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CYP3A7 antibody (H00001551-B01) by Western Blots.
SDS-PAGE Gel
CYP3A7 transfected lysate.
Western Blot
Lane 1: CYP3A7 transfected lysate ( 57.5 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CYP3A7
Entrez GeneID
1551GeneBank Accession#
NM_000765.2Protein Accession#
NP_000756.1Gene Name
CYP3A7
Gene Alias
CP37, P450-HFLA
Gene Description
cytochrome P450, family 3, subfamily A, polypeptide 7
Omim ID
605340Gene Ontology
HyperlinkGene Summary
This gene, CYP3A7, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme hydroxylates testosterone and dehydroepiandrosterone 3-sulphate, which is involved in the formation of estriol during pregnancy. The enzyme also metabolizes some drugs such as aflatoxin B1. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Transcript variants have been described, but it is not known whether these transcripts are normally produced. [provided by RefSeq
Other Designations
aryl hydrocarbon hydroxylase|cytochrome P450, subfamily IIIA, polypeptide 7|flavoprotein-linked monooxygenase|microsomal monooxygenase|xenobiotic monooxygenase
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