CYP1A1 293T Cell Transient Overexpression Lysate(Denatured)
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Specifications
Transfected Cell Line
293T
Plasmid
pCMV-CYP1A1 full-length
Host
Human
Theoretical MW (kDa)
56.43
Interspecies Antigen Sequence
Mouse (80)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CYP1A1 antibody (H00001543-B01) by Western Blots.
SDS-PAGE Gel
CYP1A1 transfected lysate.
Western Blot
Lane 1: CYP1A1 transfected lysate ( 56.43 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CYP1A1
Entrez GeneID
1543GeneBank Accession#
NM_000499.2Protein Accession#
NP_000490.1Gene Name
CYP1A1
Gene Alias
AHH, AHRR, CP11, CYP1, P1-450, P450-C, P450DX
Gene Description
cytochrome P450, family 1, subfamily A, polypeptide 1
Omim ID
108330Gene Ontology
HyperlinkGene Summary
This gene, CYP1A1, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. The gene has been associated with lung cancer risk. A related family member, CYP1A2, is located approximately 25 kb away from CYP1A1 on chromosome 15. [provided by RefSeq
Other Designations
aryl hydrocarbon hydroxylase|cytochrome P1-450, dioxin-inducible|cytochrome P450 1A1|cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 1|flavoprotein-linked monooxygenase|xenobiotic monooxygenase
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Interactomes
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Pathways
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