CTSL 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
CTSL1 transfected lysate.
Western Blot
Lane 1: CTSL1 transfected lysate ( 37.60 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CTSL full-length
Host
Human
Theoretical MW (kDa)
37.6
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CTSL antibody (H00001514-D01P) by Western Blots.
SDS-PAGE Gel
CTSL1 transfected lysate.
Western Blot
Lane 1: CTSL1 transfected lysate ( 37.60 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CTSL1
Entrez GeneID
1514GeneBank Accession#
NM_001912.2Protein Accession#
NP_001903.1Gene Name
CTSL1
Gene Alias
CATL, CTSL, FLJ31037, MEP
Gene Description
cathepsin L1
Omim ID
116880Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. At least two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000021601|OTTHUMP00000021602|OTTHUMP00000063566|cathepsin L|major excreted protein
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