CTSE 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
CTSE transfected lysate
Western Blot
Lane 1: CTSE transfected lysate ( 41.8 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CTSE full-length
Host
Human
Theoretical MW (kDa)
41.8
Interspecies Antigen Sequence
Mouse (83); Rat (77)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CTSE antibody (H00001510-B01) by Western Blots.
SDS-PAGE Gel
CTSE transfected lysate
Western Blot
Lane 1: CTSE transfected lysate ( 41.8 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CTSE
Entrez GeneID
1510GeneBank Accession#
BC042537Protein Accession#
AAH42537Gene Name
CTSE
Gene Alias
CATE
Gene Description
cathepsin E
Omim ID
116890Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a gastric aspartyl protease that functions as a disulfide-linked homodimer. This protease, which is a member of the peptidase C1 family, has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that does not appear to be involved in the digestion of dietary protein and is found in highest concentration in the surface of epithelial mucus-producing cells of the stomach. It is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of gastric cancers. It appears, therefore, to be an oncofetal antigen. Transcript variants utilizing alternative polyadenylation signals and two transcript variants encoding different isoforms exist for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000034345|OTTHUMP00000034346|erythrocyte membrane aspartic proteinase|slow-moving proteinase
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