CTSE monoclonal antibody (M10), clone 2D5
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of CTSE expression in transfected 293T cell line by CTSE monoclonal antibody (M10), clone 2D5.
Lane 1: CTSE transfected lysate (Predicted MW: 42.8 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CTSE is 1 ng/ml as a capture antibody.
Western Blot detection against Immunogen (67.43 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant CTSE.
Immunogen
CTSE (AAH42537, 18 a.a. ~ 396 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
QGSLHRVPLRRHPTLKKKLRARSQLSEFWKSHNLDMIQFTESCSMDQSAKEPLINYLDMEYFGTISIGSPPQNFTVIFDTGSSNLWVPSVYCTSPACKTHSRFQPSQSSTYSQPGQSFSIQYGTGSLSGIIGADQVSVEGLTVVGQQFGESVTEPGQTLVDAEFDGILGLGYPSLAVGGVTPVFDNMMAQNLVDLPMFSVYMSSNPEGGAGSELIFGGYDHSHFSGSLNWVPVTKQAYWQIALDNIQVGGTVMFCSEGCQAIVDTGTSLITGPSDKIKQLQNAIGAAPVDGEYAVECANLNVMPDVTFTINGVPYTLSPTAYTLLDFVDGMQFCSSGFQGLDIHPPAGPLWILGDVFIRQFYSVFDRGNNRVGLAPAVP
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (83); Rat (77)
Isotype
IgG2a
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (67.43 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of CTSE expression in transfected 293T cell line by CTSE monoclonal antibody (M10), clone 2D5.
Lane 1: CTSE transfected lysate (Predicted MW: 42.8 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CTSE is 1 ng/ml as a capture antibody.ELISA
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Gene Info — CTSE
Entrez GeneID
1510GeneBank Accession#
BC042537Protein Accession#
AAH42537Gene Name
CTSE
Gene Alias
CATE
Gene Description
cathepsin E
Omim ID
116890Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a gastric aspartyl protease that functions as a disulfide-linked homodimer. This protease, which is a member of the peptidase C1 family, has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that does not appear to be involved in the digestion of dietary protein and is found in highest concentration in the surface of epithelial mucus-producing cells of the stomach. It is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of gastric cancers. It appears, therefore, to be an oncofetal antigen. Transcript variants utilizing alternative polyadenylation signals and two transcript variants encoding different isoforms exist for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000034345|OTTHUMP00000034346|erythrocyte membrane aspartic proteinase|slow-moving proteinase
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Interactome
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Pathway
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