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NKX2-5 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00001482-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-NKX2-5 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 34.9
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-NKX2-5 antibody (H00001482-D01P) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00001482-T01
    NKX2-5 transfected lysate.
    Western Blot
    QC Testing of H00001482-T01
    Lane 1: NKX2-5 transfected lysate ( 35.75 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 1482
  • Gene Name:
  • NKX2-5
  • Gene Alias:
  • CHNG5,CSX,CSX1,NKX2.5,NKX2E,NKX4-1
  • Gene Description:
  • NK2 transcription factor related, locus 5 (Drosophila)
  • Gene Summary:
  • Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development (Shiojima et al., 1995 [PubMed 7665173]). It has been demonstrated that a Drosophila homeobox-containing gene called 'tinman' is expressed in the developing dorsal vessel and in the equivalent of the vertebrate heart. Mutations in tinman result in loss of heart formation in the embryo, suggesting that tinman is essential for Drosophila heart formation. Furthermore, abundant expression of Csx, the presumptive mouse homolog of tinman, is observed only in the heart from the time of cardiac differentiation. CSX, the human homolog of murine Csx, has a homeodomain sequence identical to that of Csx and is expressed only in the heart, again suggesting that CSX plays an important role in human heart formation.[supplied by OMIM
  • Other Designations:
  • NK2 transcription factor homolog E,NK2 transcription factor related, locus 5,cardiac-specific homeo box,tinman paralog
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