This antibody pair set comes with matched antibody pair to detect and quantify protein level of human NKX2-5.
Quality Control Testing:
Standard curve using recombinant protein ( H00001482-P01 ) as an analyte.
Sandwich ELISA detection sensitivity ranging from 3 ng/ml to 100 ng/ml.
Antibody pair set content: 1. Capture antibody: rabbit MaxPab® affinity purified polyclonal anti-NKX2-5 (100 ug) 2. Detection antibody: mouse monoclonal anti-NKX2-5, IgG1 Kappa (20 ug) *Reagents are sufficient for at least 1-2 x 96 well plates using recommended protocols.
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development (Shiojima et al., 1995 [PubMed 7665173]). It has been demonstrated that a Drosophila homeobox-containing gene called 'tinman' is expressed in the developing dorsal vessel and in the equivalent of the vertebrate heart. Mutations in tinman result in loss of heart formation in the embryo, suggesting that tinman is essential for Drosophila heart formation. Furthermore, abundant expression of Csx, the presumptive mouse homolog of tinman, is observed only in the heart from the time of cardiac differentiation. CSX, the human homolog of murine Csx, has a homeodomain sequence identical to that of Csx and is expressed only in the heart, again suggesting that CSX plays an important role in human heart formation.[supplied by OMIM