Products

Western Blot (Transfected lysate)

Western Blot analysis of CSTF3 expression in transfected 293T cell line by CSTF3 monoclonal antibody (M01), clone 1D4.

Lane 1: CSTF3 transfected lysate(11.44 KDa).
Lane 2: Non-transfected lysate.

Western Blot (Recombinant protein)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunoperoxidase of monoclonal antibody to CSTF3 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]

Sandwich ELISA (Recombinant protein)

Detection limit for recombinant GST tagged CSTF3 is approximately 0.1ng/ml as a capture antibody.

ELISA

RNAi Knockdown (Antibody validated)

Western blot analysis of CSTF3 over-expressed 293 cell line, cotransfected with CSTF3 Validated Chimera RNAi ( Cat # H00001479-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CSTF3 monoclonal antibody (M01), clone 1D4 (Cat # H00001479-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Immunofluorescence

Immunofluorescence of monoclonal antibody to CSTF3 on HeLa cell. [antibody concentration 10 ug/ml]

Entrez GeneID

1479

GeneBank Accession#

BC009792

Protein Accession#

AAH09792

Gene Name

CSTF3

Gene Alias

CSTF-77, MGC117398, MGC43001, MGC75122

Gene Description

cleavage stimulation factor, 3' pre-RNA, subunit 3, 77kDa

Omim ID

600367

Gene Ontology

Hyperlink

Gene Summary

The protein encoded by this gene is one of three (including CSTF1 and CSTF2) cleavage stimulation factors that combine to form the cleavage stimulation factor complex (CSTF). This complex is involved in the polyadenylation and 3' end cleavage of pre-mRNAs. The encoded protein functions as a homodimer and interacts directly with both CSTF1 and CSTF2 in the CSTF complex. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq

Other Designations

CSTF 77 kDa subunit|cleavage stimulation factor subunit 3|cleavage stimulation factor subunit 3, isoform 1