CSTB 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
CSTB transfected lysate.
Western Blot
Lane 1: CSTB transfected lysate ( 10.89 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CSTB full-length
Host
Human
Theoretical MW (kDa)
10.89
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CSTB antibody (H00001476-B02) by Western Blots.
SDS-PAGE Gel
CSTB transfected lysate.
Western Blot
Lane 1: CSTB transfected lysate ( 10.89 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CSTB
Entrez GeneID
1476GeneBank Accession#
NM_000100.2Protein Accession#
-Gene Name
CSTB
Gene Alias
CST6, EPM1, PME, STFB
Gene Description
cystatin B (stefin B)
Gene Ontology
HyperlinkGene Summary
The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). [provided by RefSeq
Other Designations
CPI-B|cystatin B|liver thiol proteinase inhibitor|stefin B
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Interactome
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Disease
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