CRYZ 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CRYZ full-length
Host
Human
Theoretical MW (kDa)
36.3
Interspecies Antigen Sequence
Mouse (81); Rat (81)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CRYZ antibody (H00001429-B01) by Western Blots.
SDS-PAGE Gel
CRYZ transfected lysate.
Western Blot
Lane 1: CRYZ transfected lysate ( 36.3 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CRYZ
Entrez GeneID
1429GeneBank Accession#
NM_001889.2Protein Accession#
NP_001880.2Gene Name
CRYZ
Gene Alias
DKFZp779E0834, FLJ41475
Gene Description
crystallin, zeta (quinone reductase)
Omim ID
123691Gene Ontology
HyperlinkGene Summary
Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. The former class is also called phylogenetically-restricted crystallins. This gene encodes a taxon-specific crystallin protein which has NADPH-dependent quinone reductase activity distinct from other known quinone reductases. It lacks alcohol dehydrogenase activity although by similarity it is considered a member of the zinc-containing alcohol dehydrogenase family. Unlike other mammalian species, in humans, lens expression is low. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One pseudogene is known to exist. [provided by RefSeq
Other Designations
NADPH:quinone reductase|OTTHUMP00000011194|crystallin, zeta|quinone oxidoreductase
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