CRKL 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CRKL full-length
Host
Human
Theoretical MW (kDa)
33.44
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CRKL antibody (H00001399-B01) by Western Blots.
Western Blot
Lane 1: CRKL transfected lysate ( 33.8 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
CRKL transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CRKL
Entrez GeneID
1399GeneBank Accession#
NM_005207Protein Accession#
NP_005198Gene Name
CRKL
Gene Alias
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Gene Description
v-crk sarcoma virus CT10 oncogene homolog (avian)-like
Omim ID
602007Gene Ontology
HyperlinkGene Summary
This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic
Other Designations
v-crk avian sarcoma virus CT10 oncogene homolog-like
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Interactome
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Pathway
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Disease
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