CRK 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
CRK transfected lysate.
Western Blot
Lane 1: CRK transfected lysate ( 22.90 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CRK full-length
Host
Human
Theoretical MW (kDa)
22.9
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CRK antibody (H00001398-B01) by Western Blots.
SDS-PAGE Gel
CRK transfected lysate.
Western Blot
Lane 1: CRK transfected lysate ( 22.90 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CRK
Entrez GeneID
1398GeneBank Accession#
NM_005206Protein Accession#
NP_005197.3Gene Name
CRK
Gene Alias
CRKII
Gene Description
v-crk sarcoma virus CT10 oncogene homolog (avian)
Omim ID
164762Gene Ontology
HyperlinkGene Summary
This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq
Other Designations
avian sarcoma virus CT10 (v-crk) oncogene homolog|v-crk avian sarcoma virus CT10 oncogene homolog|v-crk sarcoma virus CT10 oncogene homolog
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Interactome
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Pathway
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