COX11 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-COX11 full-length
Host
Human
Theoretical MW (kDa)
30.47
Interspecies Antigen Sequence
Mouse (83); Rat (83)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-COX11 antibody (H00001353-B02) by Western Blots.
SDS-PAGE Gel
COX11 transfected lysate.
Western Blot
Lane 1: COX11 transfected lysate ( 30.47 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — COX11
Entrez GeneID
1353GeneBank Accession#
BC005895Protein Accession#
AAH05895Gene Name
COX11
Gene Alias
COX11P
Gene Description
COX11 homolog, cytochrome c oxidase assembly protein (yeast)
Omim ID
603648Gene Ontology
HyperlinkGene Summary
Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes a protein which is not a structural subunit, but may be a heme A biosynthetic enzyme involved in COX formation, according to the yeast mutant studies. However, the studies in Rhodobacter sphaeroides suggest that this gene is not required for heme A biosynthesis, but required for stable formation of the Cu(B) and magnesium centers of COX. This human protein is predicted to contain a transmembrane domain localized in the mitochondrial inner membrane. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene has been found on chromosome 6. [provided by RefSeq
Other Designations
COX11 homolog|cytochrome c oxidase assembly protein COX11|cytochrome c oxidase subunit 11
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