TPP1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
TPP1 transfected lysate.
Western Blot
Lane 1: TPP1 transfected lysate ( 62.04 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-TPP1 full-length
Host
Human
Theoretical MW (kDa)
62.04
Interspecies Antigen Sequence
Mouse (88)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-TPP1 antibody (H00001200-B01) by Western Blots.
SDS-PAGE Gel
TPP1 transfected lysate.
Western Blot
Lane 1: TPP1 transfected lysate ( 62.04 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — TPP1
Entrez GeneID
1200GeneBank Accession#
NM_000391Protein Accession#
NP_000382Gene Name
TPP1
Gene Alias
CLN2, GIG1, LPIC, MGC21297
Gene Description
tripeptidyl peptidase I
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the sedolisin family of serine proteases. The protease functions in the lysosome to cleave N-terminal tripeptides from substrates, and has weaker endopeptidase activity. It is synthesized as a catalytically-inactive enzyme which is activated and auto-proteolyzed upon acidification. Mutations in this gene result in late-infantile neuronal ceroid lipofuscinosis, which is associated with the failure to degrade specific neuropeptides and a subunit of ATP synthase in the lysosome. [provided by RefSeq
Other Designations
ceroid-lipofuscinosis, neuronal 2, late infantile (Jansky-Bielschowsky disease)|growth-inhibiting protein 1|lysosomal pepstatin insensitive protease|tripeptidyl aminopeptidase|tripeptidyl-peptidase I
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Interactome
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Pathway
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Disease
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