CLIC1 monoclonal antibody (M01), clone 2D4
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More Files
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Western Blot (Cell lysate)
CLIC1 monoclonal antibody (M01), clone 2D4 Western Blot analysis of CLIC1 expression in HL-60 ( Cat # L014V1 ).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to CLIC1 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 3 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CLIC1 is 1 ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to CLIC1 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (52.25 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant CLIC1.
Immunogen
CLIC1 (AAH64527.1, 1 a.a. ~ 241 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAEEQPQVELFVKAGSDGAKIGNCPFSQRLFMVLWLKGVTFNVTTVDTKRRTETVQKLCPGGQLPFLLYGTEVHTDTNKIEEFLEAVLCPPRYPKLAALNPESNTAGLDIFAKFSAYIKNSSPALNDNLEKGLLKALKVLDNYLTSPLPEEVDETSAEDEGVSQRKFLDGNELTLADCNLLPKLHIVQVVCKKYRGFTIPEAFRGVHRYLSNAYAREEFASTCPDDEEIELAYEQVAKALK
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (52.25 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
CLIC1 monoclonal antibody (M01), clone 2D4 Western Blot analysis of CLIC1 expression in HL-60 ( Cat # L014V1 ).Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to CLIC1 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CLIC1 is 1 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to CLIC1 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — CLIC1
Entrez GeneID
1192GeneBank Accession#
BC064527Protein Accession#
AAH64527.1Gene Name
CLIC1
Gene Alias
G6, NCC27
Gene Description
chloride intracellular channel 1
Omim ID
602872Gene Ontology
HyperlinkGene Summary
Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 1 is a member of the p64 family; the protein localizes principally to the cell nucleus and exhibits both nuclear and plasma membrane chloride ion channel activity. [provided by RefSeq
Other Designations
OTTHUMP00000029131|OTTHUMP00000029133|OTTHUMP00000029137|OTTHUMP00000174486|RNCC protein|chloride channel ABP|nuclear chloride ion channel protein|p64CLCP
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Interactome
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Disease
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Publication Reference
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Redox proteomics reveal stress responsive proteins linking peroxiredoxin-1 status in glioma to chemosensitivity and oxidative stress.
Poschmann G, Grzendowski M, Stefanski A, Bruns E, Meyer HE, Stuhler K.
Biochimica et Biophysica Acta 2015 Jun; 1854(6):624.
Application：WB, Human, A-172 cells.
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Proteomic differences between hepatocellular carcinoma and non-tumorous liver tissue investigated by a combined 2D-DIGE and label-free quantitative proteomics study.
Megger DA, Bracht T, Kohl M, Ahrens M, Naboulsi W, Weber F, Hoffmann AC, Stephan C, Kuhlmann K, Eisenacher M, Schlaak JF, Baba HA, Meyer HE, Sitek B.
Molecular & Cellular Proteomics 2013 Jul; 12(7):2006.
Application：WB, IHC, Human, human liver carcinoma.
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Redox proteomics reveal stress responsive proteins linking peroxiredoxin-1 status in glioma to chemosensitivity and oxidative stress.
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