CLCN2 monoclonal antibody (M01), clone 3E1
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More Files
- More Functions
Western Blot (Cell lysate)
CLCN2 monoclonal antibody (M01), clone 3E1 Western Blot analysis of CLCN2 expression in Hela S3 NE ( Cat # L013V3 ).
Western Blot (Transfected lysate)
Western Blot analysis of CLCN2 expression in transfected 293T cell line by CLCN2 monoclonal antibody (M01), clone 3E1.
Lane 1: CLCN2 transfected lysate(96.8 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CLCN2 is 0.3 ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of CLCN2 over-expressed 293 cell line, cotransfected with CLCN2 Validated Chimera RNAi ( Cat # H00001181-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CLCN2 monoclonal antibody (M01), clone 3E1 (Cat # H00001181-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot detection against Immunogen (64.24 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant CLCN2.
Immunogen
CLCN2 (AAH21578.1, 38 a.a. ~ 387 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
TVSTAVIVFELTGQIAHILPVMIVVILANAVAQSLQPSLYDSIIRIKKLPYLPELGWGRHQQYRVRVEDIMVRDVPHVALSCTFRDLRLALHRTKGRMLALVESPESMILLGSIERSQVVALLGAQLSPARRRQHMQERRATQTSPLSDQEGPPTPEASVCFQVNTEDSAFPAARGETHKPLKPALKRGPSVTRNLGESPTGSAESAGIALRSLFCGSPPPEAASEKLESCEKRKLKRVRISLASDADLEGEMSPEETHTIFSLLGVDHAYVTSIGRLIGIVTLKELRKAIEGSVTAQGVKVRPPLASFRDSATSSSDTETTEVHALWGPHSRHGLPREGSPSDSDDKCQ
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (64.24 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
CLCN2 monoclonal antibody (M01), clone 3E1 Western Blot analysis of CLCN2 expression in Hela S3 NE ( Cat # L013V3 ).Western Blot (Transfected lysate)
Western Blot analysis of CLCN2 expression in transfected 293T cell line by CLCN2 monoclonal antibody (M01), clone 3E1.
Lane 1: CLCN2 transfected lysate(96.8 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CLCN2 is 0.3 ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of CLCN2 over-expressed 293 cell line, cotransfected with CLCN2 Validated Chimera RNAi ( Cat # H00001181-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CLCN2 monoclonal antibody (M01), clone 3E1 (Cat # H00001181-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — CLCN2
Entrez GeneID
1181GeneBank Accession#
BC021578Protein Accession#
AAH21578.1Gene Name
CLCN2
Gene Alias
CIC-2, CLC2, ECA3, EGI3, EGMA
Gene Description
chloride channel 2
Gene Ontology
HyperlinkGene Summary
idiopathic generalized
Other Designations
Epilepsy, idiopathic generalized, susceptibility to
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Interactome
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Disease
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