AP1S1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
AP1S1 transfected lysate.
Western Blot
Lane 1: AP1S1 transfected lysate ( 18.7 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-AP1S1 full-length
Host
Human
Theoretical MW (kDa)
18.7
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-AP1S1 antibody (H00001174-B02P) by Western Blots.
SDS-PAGE Gel
AP1S1 transfected lysate.
Western Blot
Lane 1: AP1S1 transfected lysate ( 18.7 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — AP1S1
Entrez GeneID
1174GeneBank Accession#
NM_001283Protein Accession#
NP_001274.1Gene Name
AP1S1
Gene Alias
AP19, CLAPS1, FLJ92436, SIGMA1A, WUGSC:H_DJ0747G18.2
Gene Description
adaptor-related protein complex 1, sigma 1 subunit
Omim ID
603531Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is part of the clathrin coat assembly complex which links clathrin to receptors in coated vesicles. These vesicles are involved in endocytosis and Golgi processing. This protein, as well as beta-prime-adaptin, gamma-adaptin, and the medium (mu) chain AP47, form the AP-1 assembly protein complex located at the Golgi vesicle. [provided by RefSeq
Other Designations
AP-1 complex subunit sigma-1A|HA1 19 kDa subunit|clathrin assembly protein complex 1 sigma-1A small chain|clathrin coat assembly protein AP19|clathrin-associated/assembly/adaptor protein, small 1 (19kD)|golgi adaptor HA1/AP1 adaptin sigma-1A subunit|sigma
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Interactome
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Pathway
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