ERCC8 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Western Blot
Lane 1: ERCC8 transfected lysate ( 43.56 KDa)
Lane 2: Non-transfected lysate.
SDS-PAGE Gel
ERCC8 transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ERCC8 full-length
Host
Human
Theoretical MW (kDa)
43.67
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ERCC8 antibody (H00001161-B01) by Western Blots.
Western Blot
Lane 1: ERCC8 transfected lysate ( 43.56 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
ERCC8 transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ERCC8
Entrez GeneID
1161GeneBank Accession#
NM_000082Protein Accession#
NP_000073Gene Name
ERCC8
Gene Alias
CKN1, CSA
Gene Description
excision repair cross-complementing rodent repair deficiency, complementation group 8
Gene Ontology
HyperlinkGene Summary
This gene encodes a WD repeat protein, which interacts with Cockayne syndrome type B (CSB) protein and with p44 protein, a subunit of the RNA polymerase II transcription factor IIH. Mutations in this gene have been identified in patients with hereditary disease Cockayne syndrome (CS). CS cells are abnormally sensitive to ultraviolet radiation and are defective in the repair of transcriptionally active genes. [provided by RefSeq
Other Designations
Cockayne syndrome WD-repeat protein CSA|DNA excision repair protein ERCC-8
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Interactome
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Pathway
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Disease
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