CKMT2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CKMT2 full-length
Host
Human
Theoretical MW (kDa)
46.2
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CKMT2 antibody (H00001160-B01) by Western Blots.
SDS-PAGE Gel
CKMT2 transfected lysate.
Western Blot
Lane 1: CKMT2 transfected lysate ( 46.2 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CKMT2
Entrez GeneID
1160GeneBank Accession#
BC029140.1Protein Accession#
AAH29140.1Gene Name
CKMT2
Gene Alias
SMTCK
Gene Description
creatine kinase, mitochondrial 2 (sarcomeric)
Omim ID
123295Gene Ontology
HyperlinkGene Summary
Mitochondrial creatine kinase (MtCK) is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Sarcomeric mitochondrial creatine kinase has 80% homology with the coding exons of ubiquitous mitochondrial creatine kinase. This gene contains sequences homologous to several motifs that are shared among some nuclear genes encoding mitochondrial proteins and thus may be essential for the coordinated activation of these genes during mitochondrial biogenesis. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000147542|basic-type mitochondrial creatine kinase|sarcomeric mitochondrial creatine kinase
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