CKM 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
CKM transfected lysate.
Western Blot
Lane 1: CKM transfected lysate ( 43.10 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CKM full-length
Host
Human
Theoretical MW (kDa)
43.1
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CKM antibody (H00001158-D01P) by Western Blots.
SDS-PAGE Gel
CKM transfected lysate.
Western Blot
Lane 1: CKM transfected lysate ( 43.10 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CKM
Entrez GeneID
1158GeneBank Accession#
BC007462Protein Accession#
AAH07462.1Gene Name
CKM
Gene Alias
CKMM, M-CK
Gene Description
creatine kinase, muscle
Omim ID
123310Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq
Other Designations
creatine kinase M chain|creatine kinase-M|muscle creatine kinase
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Interactome
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Pathway
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Disease
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