CKM monoclonal antibody (M06), clone 2F3-B11
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of CKM expression in transfected 293T cell line by CKM monoclonal antibody (M06), clone 2F3-B11.
Lane 1: CKM transfected lysate (Predicted MW: 43.1 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of CKM transfected lysate using anti-CKM monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CKM MaxPab rabbit polyclonal antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to CKM on HeLa cell . [antibody concentration 40 ug/ml]
Western Blot detection against Immunogen (67.65 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant CKM.
Immunogen
CKM (AAH07462, 1 a.a. ~ 381 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MPFGNTHNKFKLNYKPEEEYPDLSKHNNHMAKVLTLELYKKLRDKETPSGFTVDDVIQTGVDNPGHPFIMTVGCVAGDEESYEVFKELFDPIISDCHGGYKPTDKHKTDLNHENLKGGDDLDPNYVLSSRVRTGRSIKGYTLPPHCSRGERRAVEKLSVEALNSLAGEFKGKYYPLKSMTEKEQQQLIDDHFLFDKPVSPLLLASGMARDWPDARGIWHNDNKSLLVWVNEEDHLRVISMEKGGNMKEVFRRFCVGLQKIEEIFKKAGHPFMWNQHLGYVLTCPSNLGTGLRGGVHVKLAHLSKHPKFEEILTRLRLQKRGTGGVDTAAVGSVFDVSNADRLGSSEVEQVQLVVDGVKLMVEMEKKLEKGQSIDDMIPAQK
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (67.65 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of CKM expression in transfected 293T cell line by CKM monoclonal antibody (M06), clone 2F3-B11.
Lane 1: CKM transfected lysate (Predicted MW: 43.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of CKM transfected lysate using anti-CKM monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CKM MaxPab rabbit polyclonal antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to CKM on HeLa cell . [antibody concentration 40 ug/ml] -
Gene Info — CKM
Entrez GeneID
1158GeneBank Accession#
BC007462Protein Accession#
AAH07462Gene Name
CKM
Gene Alias
CKMM, M-CK
Gene Description
creatine kinase, muscle
Omim ID
123310Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq
Other Designations
creatine kinase M chain|creatine kinase-M|muscle creatine kinase
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Interactome
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Pathway
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Disease
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