CKM monoclonal antibody (M02), clone 1E3
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More Files
- More Functions
Western Blot (Cell lysate)
CKM monoclonal antibody (M02), clone 1E3 Western Blot analysis of CKM expression in IMR-32 ( Cat # L008V1 ).
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CKM is 0.03 ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to CKM on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (36.74 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant CKM.
Immunogen
CKM (NP_001815, 282 a.a. ~ 381 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
TCPSNLGTGLRGGVHVKLAHLSKHPKFEEILTRLRLQKRGTGGVDTAAVGSVFDVSNADRLGSSEVEQVQLVVDGVKLMVEMEKKLEKGQSIDDMIPAQK
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
CKM monoclonal antibody (M02), clone 1E3 Western Blot analysis of CKM expression in IMR-32 ( Cat # L008V1 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CKM is 0.03 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to CKM on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — CKM
Entrez GeneID
1158GeneBank Accession#
NM_001824Protein Accession#
NP_001815Gene Name
CKM
Gene Alias
CKMM, M-CK
Gene Description
creatine kinase, muscle
Omim ID
123310Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq
Other Designations
creatine kinase M chain|creatine kinase-M|muscle creatine kinase
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Interactome
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Pathway
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Disease
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Publication Reference
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Nfix regulates fetal-specific transcription in developing skeletal muscle.
Graziella Messina, Stefano Biressi, Stefania Monteverde, Alessandro Magli, Marco Cassano, Laura Perani, Elena Roncaglia, Enrico Tagliafico, Linda Starnes, Christine E Campbell, Milena Grossi, David J Goldhamer, Richard M Gronostajski, Giulio Cossu.
Cell 2010 Feb; 140(4):554.
Application：WB-Ti, Mouse, Mouse myoblasts.
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Nfix regulates fetal-specific transcription in developing skeletal muscle.
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