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CISH 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00001154-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-CISH full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 28.7
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-CISH antibody (H00001154-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00001154-T01
    CISH transfected lysate
    Western Blot
    QC Testing of H00001154-T01
    Lane 1: CISH transfected lysate ( 28.7 KDa).
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 1154
  • Gene Name:
  • CISH
  • Gene Alias:
  • CIS,CIS-1,G18,SOCS
  • Gene Description:
  • cytokine inducible SH2-containing protein
  • Gene Summary:
  • The protein encoded by this gene contains a SH2 domain and a SOCS box domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by IL2, IL3, GM-CSF and EPO in hematopoietic cells. Proteasome-mediated degradation of this protein has been shown to be involved in the inactivation of the erythropoietin receptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • cytokine-inducible SH2-containing protein,cytokine-inducible inhibitor of signaling type 1B,suppressor of cytokine signaling
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