CHRNE monoclonal antibody (M02), clone 1H5
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant CHRNE.
Immunogen
CHRNE (NP_000071, 21 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
KNEELRLYHHLFNNYDPGSRPVREPEDTVTISLKVTLTNLISLNEKEETLTTSVWIGIDWQDYRLNYSKDDFGGIETLRVPSELVWLPEIVLENNIDGQFGVAYDANVLV
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (88); Rat (88)
Isotype
IgG3 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.84 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CHRNE is 0.3 ng/ml as a capture antibody.ELISA
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Gene Info — CHRNE
Entrez GeneID
1145GeneBank Accession#
NM_000080Protein Accession#
NP_000071Gene Name
CHRNE
Gene Alias
ACHRE, CMS1D, CMS1E, CMS2A, FCCMS, SCCMS
Gene Description
cholinergic receptor, nicotinic, epsilon
Gene Ontology
HyperlinkGene Summary
Acetylcholine receptors at mature mammalian neuromuscular junctions are pentameric protein complexes composed of four subunits in the ratio of two alpha subunits to one beta, one epsilon, and one delta subunit. The acetylcholine receptor changes subunit composition shortly after birth when the epsilon subunit replaces the gamma subunit seen in embryonic receptors. Mutations in the epsilon subunit are associated with congenital myasthenic syndrome. [provided by RefSeq
Other Designations
AchR epsilon subunit|cholinergic receptor, nicotinic, epsilon polypeptide|nicotinic acetylcholine receptor epsilon polypeptide
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Interactome
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Disease
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