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CDK4 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00001019-T02
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-CDK4 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 33.7
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-CDK4 antibody (H00001019-D01P) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00001019-T02
    CDK4 transfected lysate.
    Western Blot
    QC Testing of H00001019-T02
    Lane 1: CDK4 transfected lysate ( 33.70 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 1019
  • Gene Name:
  • CDK4
  • Gene Alias:
  • CMM3,MGC14458,PSK-J3
  • Gene Description:
  • cyclin-dependent kinase 4
  • Gene Summary:
  • The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported. [provided by RefSeq
  • Other Designations:
  • cell division kinase 4,melanoma cutaneous malignant, 3
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