CDC34 (Human) Recombinant Protein (P01)
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More Files
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Specification
Product Description
Human CDC34 full-length ORF ( NP_004350.1, 1 a.a. - 236 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MARPLVPSSQKALLLELKGLQEEPVEGFRVTLVDEGDLYNWEVAIFGPPNTYYEGGYFKARLKFPIDYPYSPPAFRFLTKMWHPNIYETGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMYRKWKESKGKDREYTDIIRKQVLGTKVDAERDGVKVPTTLAEYCVKTKAPAPDEGSDLFYDDYYEDGEVEEEADSCFGDDEDDSGTEES
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
53.1
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — CDC34
Entrez GeneID
997GeneBank Accession#
NM_004359.1Protein Accession#
NP_004350.1Gene Name
CDC34
Gene Alias
E2-CDC34, UBC3, UBE2R1
Gene Description
cell division cycle 34 homolog (S. cerevisiae)
Omim ID
116948Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication. [provided by RefSeq
Other Designations
ubiquitin carrier protein|ubiquitin-conjugating enzyme Cdc34|ubiquitin-conjugating enzyme E2-32 KDA complementing|ubiquitin-protein ligase
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Interactome
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Pathway
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Disease
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Publication Reference
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An engineered platform for reconstituting functional multisubunit SCF E3 ligase in vitro.
Huihui Liu, Simiao Liu, Hong Yu, Xiahe Huang, Yingchun Wang, Liang Jiang, Xiangbing Meng, Guifu Liu, Mingjiang Chen, Yanhui Jing, Feifei Yu, Bing Wang, Jiayang Li.
Molecular Plant 2022 Aug; 15(8):1285.
Application：enzyme reconstitution, Human, E3 ligase.
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An engineered platform for reconstituting functional multisubunit SCF E3 ligase in vitro.
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