CDC25B 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Western Blot
Lane 1: CDC25B transfected lysate ( 65 KDa)
Lane 2: Non-transfected lysate.
SDS-PAGE Gel
CDC25B transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CDC25B full-length
Host
Human
Theoretical MW (kDa)
63.91
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CDC25B antibody (H00000994-B01) by Western Blots.
Western Blot
Lane 1: CDC25B transfected lysate ( 65 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
CDC25B transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CDC25B
Entrez GeneID
994GeneBank Accession#
NM_021873Protein Accession#
NP_068659Gene Name
CDC25B
Gene Alias
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Gene Description
cell division cycle 25 homolog B (S. pombe)
Omim ID
116949Gene Ontology
HyperlinkGene Summary
CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq
Other Designations
OTTHUMP00000030137|OTTHUMP00000030138|OTTHUMP00000030139|cell division cycle 25B
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