CDC6 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
CDC6 transfected lysate.
Western Blot
Lane 1: CDC6 transfected lysate ( 62.70 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CDC6 full-length
Host
Human
Theoretical MW (kDa)
62.7
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CDC6 antibody (H00000990-D01P) by Western Blots.
SDS-PAGE Gel
CDC6 transfected lysate.
Western Blot
Lane 1: CDC6 transfected lysate ( 62.70 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CDC6
Entrez GeneID
990GeneBank Accession#
BC025232Protein Accession#
AAH25232.1Gene Name
CDC6
Gene Alias
CDC18L, HsCDC18, HsCDC6
Gene Description
cell division cycle 6 homolog (S. cerevisiae)
Omim ID
602627Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. [provided by RefSeq
Other Designations
CDC18 (cell division cycle 18, S.pombe, homolog)-like|CDC6 cell division cycle 6 homolog|CDC6-related protein|cell division cycle 6 protein
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Interactome
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Pathway
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Disease
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