CD97 monoclonal antibody (M01), clone 5D5
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of CD97 expression in transfected 293T cell line by CD97 monoclonal antibody (M01), clone 5D5.
Lane 1: CD97 transfected lysate(81.7 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CD97 is approximately 0.1ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of CD97 over-expressed 293 cell line, cotransfected with CD97 Validated Chimera RNAi ( Cat # H00000976-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CD97 monoclonal antibody (M01), clone 5D5 (Cat # H00000976-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot detection against Immunogen (37.73 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant CD97.
Immunogen
CD97 (NP_510966, 421 a.a. ~ 529 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
KKQAELEEIYESSIRGVQLRRLSAVNSIFLSHNNTKELNSPILFAFSHLESSDGEAGRDPPAKDVMPGPRQELLCAFWKSDSDRGGHWATEGCQVLGSKNGSTTCQCSH
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (38); Rat (35)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.73 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of CD97 expression in transfected 293T cell line by CD97 monoclonal antibody (M01), clone 5D5.
Lane 1: CD97 transfected lysate(81.7 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CD97 is approximately 0.1ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of CD97 over-expressed 293 cell line, cotransfected with CD97 Validated Chimera RNAi ( Cat # H00000976-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CD97 monoclonal antibody (M01), clone 5D5 (Cat # H00000976-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — CD97
Entrez GeneID
976GeneBank Accession#
NM_078481Protein Accession#
NP_510966Gene Name
CD97
Gene Alias
TM7LN1
Gene Description
CD97 molecule
Omim ID
601211Gene Ontology
HyperlinkGene Summary
This gene is a member of the EGF-TM7 family of class II seven-span transmembrane (7-TM) molecules, likely encoded by a gene cluster on the short arm of chromosome 19. The encoded product is a glycoprotein that is present on the surface of most activated leukocytes and spans the membrane seven times, which is a defining feature of G protein-coupled receptors. The protein has an extended extracellular region with several N-terminal epidermal growth factor (EGF)-like domains, which mediate binding to its cellular ligand, decay accelerating factor (DAF, CD55), a regulatory protein of the complement cascade. The presence of structural features characteristic of extracellular matrix proteins and transmembrane proteins suggests that this protein is a receptor involved in both cell adhesion and signaling processes early after leukocyte activation. Alternative splicing has been observed for this gene and three variants have been found. [provided by RefSeq
Other Designations
CD97 antigen|leukocyte antigen CD97|seven transmembrane helix receptor|seven-span transmembrane protein|seven-transmembrane, heterodimeric receptor associated with inflammation
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Interactome
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Disease
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