CD47 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
CD47 transfected lysate.
Western Blot
Lane 1: CD47 transfected lysate ( 35.2 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CD47 full-length
Host
Human
Theoretical MW (kDa)
35.2
Interspecies Antigen Sequence
Mouse (66); Rat (70)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CD47 antibody (H00000961-D01P) by Western Blots.
SDS-PAGE Gel
CD47 transfected lysate.
Western Blot
Lane 1: CD47 transfected lysate ( 35.2 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CD47
Entrez GeneID
961GeneBank Accession#
NM_001777Protein Accession#
NP_001768.1Gene Name
CD47
Gene Alias
IAP, MER6, OA3
Gene Description
CD47 molecule
Omim ID
601028Gene Ontology
HyperlinkGene Summary
This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Four alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq
Other Designations
CD47 antigen|CD47 antigen (Rh-related antigen, integrin-associated signal transducer)|CD47 glycoprotein|Rh-related antigen|antigen identified by monoclonal antibody 1D8|antigenic surface determinant protein OA3|integrin associated protein|integrin-associa
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Interactome
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Pathway
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