Western blot analysis of CD33 over-expressed 293 cell line, cotransfected with CD33 Validated Chimera RNAi ( Cat # H00000945-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CD33 monoclonal antibody (M01), clone 2D12-G4 (Cat # H00000945-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.