CD80 monoclonal antibody (M01), clone 1G1
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant CD80.
Immunogen
CD80 (AAH42665, 35 a.a. ~ 288 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
VIHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIWPEYKNRTIFDITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEVTLSVKADFPTPSISDFEIPTSNIRRIICSTSGGFPEPHLSWLENGEELNAINTTVSQDPETELYAVSSKLDFNMTTNHSFMCLIKYGHLRVNQTFNWNTTKQEHFPDNLLPSWAITLISVNGIFVICCLTYCFAPRCRERRRNERLRRESVRPV
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (47)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CD80 is 0.3 ng/ml as a capture antibody.ELISA
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between CD86 and CD80. HeLa cells were stained with anti-CD86 rabbit purified polyclonal 1:1200 and anti-CD80 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue). -
Gene Info — CD80
Entrez GeneID
941GeneBank Accession#
BC042665Protein Accession#
AAH42665Gene Name
CD80
Gene Alias
CD28LG, CD28LG1, LAB7
Gene Description
CD80 molecule
Omim ID
112203Gene Ontology
HyperlinkGene Summary
The B-lymphocyte activation antigen B7-1 (formerly referred to as B7) provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 (MIM 186760) and CTLA4 (MIM 123890) ligands of T cells.[supplied by OMIM
Other Designations
B-lymphocyte activation antigen B7|CD80 antigen|CD80 antigen (CD28 antigen ligand 1, B7-1 antigen)|costimulatory factor CD80|costimulatory molecule variant IgV-CD80
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Interactome
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Pathway
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Disease
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