CD28 (Human) Recombinant Protein
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Specification
Product Description
Purified CD28 (NP_006130.1, 18 a.a. - 152 a.a.) human recombinant protein with His-Flag-StrepII tag at N-terminus expressed in human cells.
Transfected Cell Line
Human HEK293H cells
Sequence
GNKILVKQSPMLVAYDNAVNLSCKYSYNLFSREFRASLHKGLDSAVEVCVVYGNYSQQLQVYSKTGFNCDGKLGNESVTFYLQNLYVNQTDIYFCKIEVMYPPPYLDNEKSNGTIIHVKGKHLCPSPLFPGPSKP
Host
Human
Theoretical MW (kDa)
20.13
Interspecies Antigen Sequence
Mouse (70)
Form
Liquid
Preparation Method
Transfection of pSuper-CD28 plasmid into HEK293H cell, and the expressed protein was purified by Strep-Tactin affinity column.
Purification
Strep-Tactin affinity columns
Concentration
≥ 10 ug/ml
Storage Buffer
100 mM Tris-HCl pH 8.0, 150 mM NaCl, 1 mM EDTA, and 5 mM desthiobiotin.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Enzyme-linked Immunoabsorbent Assay
SDS-PAGE
Protein Interaction
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Gene Info — CD28
Entrez GeneID
940GeneBank Accession#
NM_006139.3Protein Accession#
NP_006130.1Gene Name
CD28
Gene Alias
MGC138290, Tp44
Gene Description
CD28 molecule
Omim ID
186760Gene Ontology
HyperlinkGene Summary
CD28 costimulation is essential for CD4 (MIM 186940)-positive T-cell proliferation, survival, interleukin-2 (IL2; MIM 147680) production, and T-helper type-2 (Th2) development.[supplied by OMIM
Other Designations
CD28 antigen|CD28 antigen (Tp44)|T-cell-specific surface glycoprotein
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Interactome
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Pathway
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Disease
- Acute Disease
- Arthritis
- Asthma
- Behcet Syndrome
- Bronchiolitis
- Carcinoma
- Celiac Disease
- Cholangitis
- Colon cancer
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