TNFRSF7 monoclonal antibody (M01), clone 1E2-A3
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More Files
- More Functions
Western Blot (Cell lysate)
TNFRSF7 monoclonal antibody (M01), clone 1E2-A3 Western Blot analysis of TNFRSF7 expression in Jurkat ( Cat # L017V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of TNFRSF7 expression in transfected 293T cell line by TNFRSF7 monoclonal antibody (M01), clone 1E2-A3.
Lane 1: TNFRSF7 transfected lysate(29.2 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CD27 is 0.3 ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of TNFRSF7 over-expressed 293 cell line, cotransfected with TNFRSF7 Validated Chimera RNAi ( Cat # H00000939-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TNFRSF7 monoclonal antibody (M01), clone 1E2-A3 (Cat # H00000939-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot detection against Immunogen (54.34 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant TNFRSF7.
Immunogen
TNFRSF7 (AAH12160, 1 a.a. ~ 260 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MARPHPWWLCVLGTLVGLSATPAPKSCPERHYWAQGKLCCQMCEPGTFLVKDCDQHRKAAQCDPCIPGVSFSPDHHTRPHCESCRHCNSGLLVRNCTITANAECACRNGWQCRDKECTECDPLPNPSLTARSSQALSPHPQPTHLPYVSEMLEARTAGHMQTLADFRQLPARTLSTHWPPQRSLCSSDFIRILVIFSGMFLVFTLAGALFLHQRRKYRSNKGESPVEPAEPCRYSCPREEEGSTIPIQEDYRKPEPACSP
Host
Mouse
Reactivity
Human
Isotype
IgG2b kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (54.34 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
TNFRSF7 monoclonal antibody (M01), clone 1E2-A3 Western Blot analysis of TNFRSF7 expression in Jurkat ( Cat # L017V1 ).Western Blot (Transfected lysate)
Western Blot analysis of TNFRSF7 expression in transfected 293T cell line by TNFRSF7 monoclonal antibody (M01), clone 1E2-A3.
Lane 1: TNFRSF7 transfected lysate(29.2 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CD27 is 0.3 ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of TNFRSF7 over-expressed 293 cell line, cotransfected with TNFRSF7 Validated Chimera RNAi ( Cat # H00000939-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TNFRSF7 monoclonal antibody (M01), clone 1E2-A3 (Cat # H00000939-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — CD27
Entrez GeneID
939GeneBank Accession#
BC012160Protein Accession#
AAH12160Gene Name
CD27
Gene Alias
MGC20393, S152, T14, TNFRSF7, Tp55
Gene Description
CD27 molecule
Omim ID
186711Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. [provided by RefSeq
Other Designations
CD27 antigen|CD27L receptor|T cell activation antigen CD27|T cell antivation antigen S152|tumor necrosis factor receptor superfamily, member 7
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Interactome
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Pathway
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Disease
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