CD8B 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Western Blot
Lane 1: CD8B transfected lysate ( 27.2 KDa)
Lane 2: Non-transfected lysate.
SDS-PAGE Gel
CD8B transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CD8B full-length
Host
Human
Theoretical MW (kDa)
26.84
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CD8B antibody (H00000926-B01) by Western Blots.
Western Blot
Lane 1: CD8B transfected lysate ( 27.2 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
CD8B transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CD8B
Entrez GeneID
926GeneBank Accession#
NM_172213Protein Accession#
NP_757362Gene Name
CD8B
Gene Alias
CD8B1, LYT3, Leu2, Ly3, MGC119115
Gene Description
CD8b molecule
Omim ID
186730Gene Ontology
HyperlinkGene Summary
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified. [provided by RefSeq
Other Designations
CD8 antigen, beta polypeptide (p37)|CD8 antigen, beta polypeptide 1 (p37)|CD8b antigen|OTTHUMP00000160761|T lymphocyte surface glycoprotein beta chain|T-cell surface glycoprotein CD8 beta chain
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Interactome
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Disease
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