CD3D 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Western Blot
Lane 1: CD3D transfected lysate ( 18.9 KDa)
Lane 2: Non-transfected lysate.
SDS-PAGE Gel
CD3D transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CD3D full-length
Host
Human
Theoretical MW (kDa)
18.92
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CD3D antibody (H00000915-B01) by Western Blots.
Western Blot
Lane 1: CD3D transfected lysate ( 18.9 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
CD3D transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CD3D
Entrez GeneID
915GeneBank Accession#
NM_000732Protein Accession#
NP_000723Gene Name
CD3D
Gene Alias
CD3-DELTA, T3D
Gene Description
CD3d molecule, delta (CD3-TCR complex)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined. [provided by RefSeq
Other Designations
CD3D antigen, delta polypeptide|CD3d antigen, delta polypeptide (TiT3 complex)|T-cell receptor T3 delta chain|T-cell surface glycoprotein CD3 delta chain
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Interactome
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Pathway
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Disease
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