CCNT1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
CCNT1 transfected lysate.
Western Blot
Lane 1: CCNT1 transfected lysate ( 80.7 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pV5-CCNT1 full-length
Host
Human
Theoretical MW (kDa)
80.7
Interspecies Antigen Sequence
Mouse (90); Rat (78)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CCNT1 antibody (H00000904-B02P) by Western Blots.
SDS-PAGE Gel
CCNT1 transfected lysate.
Western Blot
Lane 1: CCNT1 transfected lysate ( 80.7 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CCNT1
Entrez GeneID
904GeneBank Accession#
NM_001240Protein Accession#
NP_001231.2Gene Name
CCNT1
Gene Alias
CCNT, CYCT1
Gene Description
cyclin T1
Omim ID
602506Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin tightly associates with CDK9 kinase, and was found to be a major subunit of the transcription elongation factor p-TEFb. The kinase complex containing this cyclin and the elongation factor can interact with, and act as a cofactor of human immunodeficiency virus type 1 (HIV-1) Tat protein, and was shown to be both necessary and sufficient for full activation of viral transcription. This cyclin and its kinase partner were also found to be involved in the phosphorylation and regulation of the carboxy-terminal domain (CTD) of the largest RNA polymerase II subunit. [provided by RefSeq
Other Designations
CDK9-associated C-type protein|cyclin C-related protein|cyclin T1b|subunit of positive elongation transcription factor b
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Interactome
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Disease
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