CCNF 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
CCNF transfected lysate.
Western Blot
Lane 1: CCNF transfected lysate ( 86.57 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CCNF full-length
Host
Human
Theoretical MW (kDa)
86.57
Interspecies Antigen Sequence
Mouse (84); Rat (85)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CCNF antibody (H00000899-B01) by Western Blots.
SDS-PAGE Gel
CCNF transfected lysate.
Western Blot
Lane 1: CCNF transfected lysate ( 86.57 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CCNF
Entrez GeneID
899GeneBank Accession#
BC012349Protein Accession#
AAH12349Gene Name
CCNF
Gene Alias
FBX1, FBXO1
Gene Description
cyclin F
Omim ID
600227Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the cyclin family. Cyclins are important regulators of cell cycle transitions through their ability to bind and activate cyclin-dependent protein kinases. This member also belongs to the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and it was one of the first proteins in which the F-box motif was identified. [provided by RefSeq
Other Designations
F-box only protein 1
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