CCNB1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CCNB1 full-length
Host
Human
Theoretical MW (kDa)
48.3
Interspecies Antigen Sequence
Rat (85)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CCNB1 antibody (H00000891-B01) by Western Blots.
SDS-PAGE Gel
CCNB1 transfected lysate.
Western Blot
Lane 1: CCNB1 transfected lysate ( 48.30 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CCNB1
Entrez GeneID
891GeneBank Accession#
NM_031966Protein Accession#
NP_114172.1Gene Name
CCNB1
Gene Alias
CCNB
Gene Description
cyclin B1
Omim ID
123836Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq
Other Designations
G2/mitotic-specific cyclin B1
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Interactome
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Pathway
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Disease
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