CBR3 monoclonal antibody (M05), clone 1G8
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant CBR3.
Immunogen
CBR3 (NP_001227, 180 a.a. ~ 277 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
KNEVHEREGWPNSPYGVSKLGVTVLSRILARRLDEKRKADRILVNACCPGPVKTDMDGKDSIRTVEEGAETPVYLALLPPDATEPQGQLVHDKVVQNW
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.52 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
CBR3 monoclonal antibody (M05), clone 1G8. Western Blot analysis of CBR3 expression in HepG2(Cat # L019V1 ).Western Blot (Transfected lysate)
Western Blot analysis of CBR3 expression in transfected 293T cell line by CBR3 monoclonal antibody (M05), clone 1G8.
Lane 1: CBR3 transfected lysate (Predicted MW: 30.9 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CBR3 is 0.3 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to CBR3 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — CBR3
Entrez GeneID
874GeneBank Accession#
NM_001236Protein Accession#
NP_001227Gene Name
CBR3
Gene Alias
SDR21C2, hCBR3
Gene Description
carbonyl reductase 3
Omim ID
603608Gene Ontology
HyperlinkGene Summary
Carbonyl reductase 3 catalyzes the reduction of a large number of biologically and pharmacologically active carbonyl compounds to their corresponding alcohols. The enzyme is classified as a monomeric NADPH-dependent oxidoreductase. CBR3 contains three exons spanning 11.2 kilobases and is closely linked to another carbonyl reductase gene - CBR1. [provided by RefSeq
Other Designations
NADPH-dependent carbonyl reductase 3|carbonyl reductase (NADPH) 3|short chain dehydrogenase/reductase family 21C, member 2
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Interactome
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Pathway
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Disease
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