CASP7 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CASP7 full-length
Host
Human
Theoretical MW (kDa)
33.44
Interspecies Antigen Sequence
Mouse (82); Rat (85)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CASP7 antibody (H00000840-B01) by Western Blots.
Western Blot
Lane 1: CASP7 transfected lysate ( 34.3 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
CASP7 transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CASP7
Entrez GeneID
840GeneBank Accession#
NM_001227Protein Accession#
NP_001218Gene Name
CASP7
Gene Alias
CMH-1, ICE-LAP3, MCH3
Gene Description
caspase 7, apoptosis-related cysteine peptidase
Omim ID
601761Gene Ontology
HyperlinkGene Summary
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of this caspase is cleaved by caspase 3 and 10. It is activated upon cell death stimuli and induces apoptosis. Alternative splicing results in four transcript variants, encoding three distinct isoforms. [provided by RefSeq
Other Designations
ICE-like apoptotic protease 3|Lice2 alpha/beta/gamma|OTTHUMP00000020510|OTTHUMP00000020511|OTTHUMP00000020512|OTTHUMP00000020514|apoptotic protease MCH-3|caspase 7|caspase 7 isoform delta|caspase 7, apoptosis-related cysteine protease
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Interactome
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Pathway
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Disease
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