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Last updated: 2017/11/19

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CARS 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00000833-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-CARS full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 82.39
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-CARS antibody (H00000833-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00000833-T01
    CARS transfected lysate.
    Western Blot
    QC Testing of H00000833-T01
    Lane 1: CARS transfected lysate ( 82.39 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 833
  • Gene Name:
  • CARS
  • Gene Alias:
  • CARS1,CYSRS,MGC:11246
  • Gene Description:
  • cysteinyl-tRNA synthetase
  • Gene Summary:
  • This gene encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000012605,cysteine tRNA ligase 1, cytoplasmic,cysteine translase,cysteine-tRNA ligase
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