CARS 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
CARS transfected lysate.
Western Blot
Lane 1: CARS transfected lysate ( 82.39 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CARS full-length
Host
Human
Theoretical MW (kDa)
82.39
Interspecies Antigen Sequence
Mouse (90); Rat (90)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CARS antibody (H00000833-B01) by Western Blots.
SDS-PAGE Gel
CARS transfected lysate.
Western Blot
Lane 1: CARS transfected lysate ( 82.39 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CARS
Entrez GeneID
833GeneBank Accession#
NM_001751.4Protein Accession#
NP_001742.1Gene Name
CARS
Gene Alias
CARS1, CYSRS, MGC:11246
Gene Description
cysteinyl-tRNA synthetase
Omim ID
123859Gene Ontology
HyperlinkGene Summary
This gene encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq
Other Designations
OTTHUMP00000012605|cysteine tRNA ligase 1, cytoplasmic|cysteine translase|cysteine-tRNA ligase
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Interactome
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Pathway
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Disease
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