CAST 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
CAST transfected lysate.
Western Blot
Lane 1: CAST transfected lysate ( 74.10 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CAST full-length
Host
Human
Theoretical MW (kDa)
74.1
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CAST antibody (H00000831-D01P) by Western Blots.
SDS-PAGE Gel
CAST transfected lysate.
Western Blot
Lane 1: CAST transfected lysate ( 74.10 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CAST
Entrez GeneID
831GeneBank Accession#
NM_173060.2Protein Accession#
NP_775083.1Gene Name
CAST
Gene Alias
BS-17, MGC9402
Gene Description
calpastatin
Omim ID
114090Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length natures of only some have been determined. [provided by RefSeq
Other Designations
OTTHUMP00000158519|OTTHUMP00000158520|calpain inhibitor|heart-type calpastatin|sperm BS-17 component
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Interactome
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Disease
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