CAPN1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Western Blot
Lane 1: CAPN1 transfected lysate ( 81.9 KDa)
Lane 2: Non-transfected lysate.
SDS-PAGE Gel
CAPN1 transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CAPN1 full-length
Host
Human
Theoretical MW (kDa)
78.65
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CAPN1 antibody (H00000823-B01) by Western Blots.
Western Blot
Lane 1: CAPN1 transfected lysate ( 81.9 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
CAPN1 transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CAPN1
Entrez GeneID
823GeneBank Accession#
NM_005186Protein Accession#
NP_005177Gene Name
CAPN1
Gene Alias
CANP, CANP1, CANPL1, muCANP, muCL
Gene Description
calpain 1, (mu/I) large subunit
Omim ID
114220Gene Ontology
HyperlinkGene Summary
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. [provided by RefSeq
Other Designations
calcium-activated neutral proteinase|calpain 1, large subunit|calpain, large polypeptide L1|cell proliferation-inducing protein 30
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