CACNB4 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
CACNB4 transfected lysate.
Western Blot
Lane 1: CACNB4 transfected lysate ( 58.2 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CACNB4 full-length
Host
Human
Theoretical MW (kDa)
58.2
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CACNB4 antibody (H00000785-B01) by Western Blots.
SDS-PAGE Gel
CACNB4 transfected lysate.
Western Blot
Lane 1: CACNB4 transfected lysate ( 58.2 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CACNB4
Entrez GeneID
785GeneBank Accession#
NM_000726.2Protein Accession#
-Gene Name
CACNB4
Gene Alias
CAB4, CACNLB4, EA5, EJM
Gene Description
calcium channel, voltage-dependent, beta 4 subunit
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the beta subunit family of voltage-dependent calcium channel complex proteins. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various versions of each of these subunits exist, either expressed from similar genes or the result of alternative splicing. The protein encoded by this locus plays an important role in calcium channel function by modulating G protein inhibition, increasing peak calcium current, controlling the alpha-1 subunit membrane targeting and shifting the voltage dependence of activation and inactivation. Certain mutations in this gene have been associated with idiopathic generalized epilepsy (IGE) and juvenile myoclonic epilepsy (JME). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
dihydropyridine-sensitive L-type, calcium channel beta-4 subunit|voltage dependent calcium channel beta 4 subunit
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Interactome
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