CA9 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
CA9 transfected lysate.
Western Blot
Lane 1: CA9 transfected lysate ( 50.6 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CA9 full-length
Host
Human
Theoretical MW (kDa)
50.6
Interspecies Antigen Sequence
Mouse (69); Rat (71)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CA9 antibody (H00000768-B01) by Western Blots.
SDS-PAGE Gel
CA9 transfected lysate.
Western Blot
Lane 1: CA9 transfected lysate ( 50.6 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CA9
Entrez GeneID
768GeneBank Accession#
NM_001216.1Protein Accession#
NP_001207.1Gene Name
CA9
Gene Alias
CAIX, MN
Gene Description
carbonic anhydrase IX
Omim ID
603179Gene Ontology
HyperlinkGene Summary
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq
Other Designations
OTTHUMP00000022773|RCC-associated protein G250|carbonic dehydratase
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