CA3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
CA3 transfected lysate.
Western Blot
Lane 1: CA3 transfected lysate ( 29.60 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CA3 full-length
Host
Human
Theoretical MW (kDa)
29.6
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CA3 antibody (H00000761-B01) by Western Blots.
SDS-PAGE Gel
CA3 transfected lysate.
Western Blot
Lane 1: CA3 transfected lysate ( 29.60 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CA3
Entrez GeneID
761GeneBank Accession#
NM_005181Protein Accession#
NP_005172.1Gene Name
CA3
Gene Alias
CAIII, Car3
Gene Description
carbonic anhydrase III, muscle specific
Omim ID
114750Gene Ontology
HyperlinkGene Summary
Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encodes carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. The gene spans 10.3 kb and contains seven exons and six introns. [provided by RefSeq
Other Designations
carbonic anhydrase III
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Interactome
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