CA1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
CA1 transfected lysate.
Western Blot
Lane 1: CA1 transfected lysate ( 28.90 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CA1 full-length
Host
Human
Theoretical MW (kDa)
28.9
Interspecies Antigen Sequence
Mouse (78); Rat (81)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CA1 antibody (H00000759-D01P) by Western Blots.
SDS-PAGE Gel
CA1 transfected lysate.
Western Blot
Lane 1: CA1 transfected lysate ( 28.90 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CA1
Entrez GeneID
759GeneBank Accession#
NM_001738.1Protein Accession#
NP_001729.1Gene Name
CA1
Gene Alias
Car1
Gene Description
carbonic anhydrase I
Omim ID
114800Gene Ontology
HyperlinkGene Summary
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA1 is closely linked to CA2 and CA3 genes on chromosome 8, and it encodes a cytosolic protein which is found at the highest level in erythrocytes. Variants of this gene have been described in some populations. Multiple alternatively spliced variants, encoding the same protein, have been identified. Transcript variants of CA1 utilizing alternative polyA_sites have been described in literature. [provided by RefSeq
Other Designations
carbonic dehydratase
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Interactome
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Pathway
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Disease
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